| 產品編號 | bsm-60446M |
| 英文名稱 | Mouse Anti-PTPN2 antibody |
| 中文名稱 | 酪氨酸蛋白磷酸酶非受體2型單克隆抗體 |
| 別 名 | PTN2_HUMAN; Tyrosine-protein phosphatase non-receptor type 2; PTPT; EC:3.1.3.48; T-cell protein-tyrosine phosphatase (TCPTP); protein tyrosine phosphatase non-receptor type 2; PTN2; TCPTP; TC-PTP; TCELLPTP; |
| 研究領域 | 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | F3H2 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-1:5000,IHC-P=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011] Subcellular Location: [Isoform 2]: Nucleus. Cytoplasm. Cell membrane. Note=Predominantly localizes to chromatin (By similarity). Able to shuttle between the nucleus and the cytoplasm and to dephosphorylate plasma membrane receptors (PubMed:9488479). Recruited by activated ITGA1 at the plasma membrane. {ECO:0000250, ECO:0000269|PubMed:9488479}. Tissue Specificity: Ubiquitously expressed. Isoform 2 is probably the major isoform. Isoform 1 is expressed in T-cells and in placenta. SWISS: P17706 Gene ID: 5771 Database links: Entrez Gene: 5771 Human Entrez Gene: 19255 Mouse SwissProt: P17706 Human SwissProt: Q06180 Mouse 去磷酸化受體蛋白酪氨酸激酶的非受體型酪氨酸特異性磷酸酶，包括INSR、EGFR、CSF1R、PDGFR。在細胞核或細胞質中也去磷酸化非受體蛋白酪氨酸激酶，如JAK1、JAK2、JAK3、Src家族激酶、STAT1、STAT3和STAT6。能夠負調控許多信號通路和生物過程，如造血，炎癥反應，細胞增殖和分化，葡萄糖穩態。在免疫系統的發育中起著多方面的重要作用，通過FYN和LCK的去磷酸化來控制T細胞的分化和活化，在T細胞受體信號轉導中發揮作用。去磷酸化CSF1R，負調控其下游信號和巨噬細胞分化。通過細胞質激酶JAK1、JAK3及其底物STAT1的去磷酸化負調控細胞因子（IL2/白介素-2和干擾素）介導的信號傳導，這些激酶在細胞因子受體下游傳播信號。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HL-60, A20
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 45-50 kDa
Observed MW: 45-50 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HepG2
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 45-50 kDa
Observed MW: 45-50 kDa
Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60446M
Tissue: Rat stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60446M
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60446M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60446M
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
