| 產品編號 | bs-10771R |
| 英文名稱 | Rabbit Anti-DHRS2 antibody |
| 中文名稱 | 脫氫酶/還原酶家族成員2抗體 |
| 別 名 | Dehydrogenase/reductase (SDR family) member 2; Dehydrogenase/reductase member 2; Dehydrogenase/reductase SDR family member 2; Dicarbonyl reductase HEP27; DHRSⅡ; Hep27; HEP27 protein; Protein D; SDR family member; SDR25C1; Short chain alcohol dehydrogenase family member; Short chain dehydrogenase/reductase family 25C member 1; DHRS2_HUMAN. |
| 研究領域 | 腫瘤 神經生物學 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DHRS2: 61-160/258 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | |
| 產品圖片 |
Protein: Hela(human) lysates at 40ug;
Primary: rabbit Anti-DARC (bs-10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:27 kD
Observed band size: 30 kD
Protein: lung(mouse) lysates at 40ug;
Primary: Anti-DHRS2 (bs-10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :27 kD
Observed band size :27 kD
Protein: liver(mouse) lysates at 30 ug;
Primary: Anti-DHRS2 (bs-10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 27 kD
Observed band size : 27/75 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(bs-10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(bs-10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
