| 產品編號 | bs-13393R |
| 英文名稱 | Rabbit Anti-QARS/Glutamyl Prolyl tRNA synthetase/ProRS antibody |
| 中文名稱 | 谷氨酰-脯氨酰-tRNA合成酶抗體 |
| 別 名 | Bifunctional aminoacyl tRNA synthetase; Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; Eprs; GLNS; Glu pro tRNA synthetase; GLUPRORS; GluRS; Glutamate tRNA ligase; Glutamatyl prolyl tRNA synthetase; Glutaminyl tRNA synthetase; Glutamyl prolyl tRNA synthetase; Glutamyl tRNA synthetase; Glutamyl-tRNA synthetase; PARS; PIG 32; PIG32; Proliferation inducing gene 32 protein; Proliferation inducing protein 32; Proline tRNA ligase; Proline--tRNA ligase; Prolyl tRNA synthetase; Prolyl-tRNA synthetase; QARS; QPRS; SYEP_HUMAN. |
| 研究領域 | 細胞生物 信號轉導 轉運蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 171kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ProRS: 651-750/1512 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. ProRS (Prolyl-tRNA synthetase), also known as EPRS, EARS, PARS, QARS, QPRS, PIG32 or GLUPRORS, is a 1,512 amino acid protein that contains three WHEP-TRS domains and belongs to both the class-I and class-II aminoacyl-tRNA synthetase family. Functioning as a component of the multisynthase complex, ProRS uses ATP to catalyze the conversion of L-glutamate and tRNA(Glu) to L-glutamyl-tRNA(Glu), as well as the conversion of L-proline and tRNA(Pro) to L-prolyl-tRNA(Pro). Function: Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Subunit: Component of the multisynthetase complex which iscomprised of a bifunctional glutamyl-prolyl-tRNA synthetase, themonospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl,arginyl, and aspartyl-tRNA synthetases as well as three auxiliaryproteins, p18, p48 and p43. Interacts with DUS2L. Component of theGAIT complex; in humans the complex assembly seems to be a two-stepprocess in which EPRS first associates with SYNCRIP to form apre-GAIT complex which is deficient in GAIT element binding. Post-translational modifications: Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells. Similarity: In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family. In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family. Contains 3 WHEP-TRS domains. SWISS: P07814 Gene ID: 5859 Database links: Entrez Gene: 5859 Human Omim: 603727 Human SwissProt: P47897 Human Unigene: 79322 Human |
| 產品圖片 |
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- QARS (bs-13393R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 171 kD
Observed band size: 171 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (bs-13393R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (bs-13393R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (bs-13393R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated(bs-13393R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
