| 產品編號 | bs-7805R |
| 英文名稱 | Rabbit Anti-KIFC1 antibody |
| 中文名稱 | 驅動蛋白家族成員C1抗體 |
| 別 名 | HSET KNSL2; KIF C1; KIFC 1; KIFC1; KIFC1_HUMAN; Kinesin family member C1; Kinesin like 2; Kinesin like protein 2; Kinesin like protein KIFC1; Kinesin related protein HSET; Kinesin-like protein 2; Kinesin-like protein KIFC1; Kinesin-related protein HSET; KNSL 2; KNSL2; Mitotic kinesin like protein 2; MKLP 2; MKLP2; RAB6 KIFL; Rabkinesin 6. |
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Specific References (1) | bs-7805R has been referenced in 1 publications.
[IF=3.905] Ya-Lan Wei. et al. Kinesin-14 KIFC1 modulates spindle assembly and chromosome segregation in mouse spermatocytes. Exp Cell Res. 2022 May;414:113095 IF ; Mouse.
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| 研究領域 | 細胞生物 細胞周期蛋白 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Cow,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIFC1: 401-500/673 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. KIFC1 is a 673 amino acid protein that belongs to the kinesin-like family of proteins. KIFC1 localizes to the nucleus and contains a C-terminal kinesin-motor domain. Functioning as a minus-end directed microtubule-dependent motor, KIFC1 works together with NuMA and cytoplasmic Dynein to organize microtubule minus ends at spindle poles. HeLa cells deficient in KIFC1 exhibit multipolar mitotic spindles, suggesting that KIFC1 is essential for bipolar spindle formation. Function: Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Subunit: Binds NUBP1 and NUBP2. Interacts with PPP1R42 (By similarity). Subcellular Location: Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. Similarity: Belongs to the kinesin-like protein family. NCD subfamily. Contains 1 kinesin-motor domain. SWISS: Q9BW19 Gene ID: 3833 Database links: Entrez Gene: 3833 Human Omim: 603763 Human SwissProt: Q9BW19 Human Unigene: 436912 Human |
| 產品圖片 |
Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-KIFC1 (bs-7805R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD
Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti-KIFC1 (bs-7805R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (KIFC1) Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIFC1) Polyclonal Antibody, Unconjugated (bs-7805R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
