| 產品編號 | bs-1319R |
| 英文名稱 | Rabbit Anti-MAPK4 antibody |
| 中文名稱 | 絲裂原活化蛋白激酶4抗體 |
| 別 名 | Mitogen-activated protein kinase 4; Extracellular signal-regulated kinase 4; ERK-4; MAP kinase isoform p63; p63-MAPK; ERK-4; ERK3; Erk3 related; ERK4; Extracellular signal regulated kinase 4; MAP kinase 4; MAPK 4; MAPK4; Mitogen activated protein kinase 4; MK04_HUMAN; p63 MAPK; p63MAPK; PRKM4; A330097D03Rik; Erk3; p63Mapk; Prkm4. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 66kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAPK4: 21-130/587 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets. [provided by RefSeq, Jul 2008] Function: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity). Subunit: Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5. Tissue Specificity: High expression in heart and brain. Post-translational modifications: Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. SWISS: P31152 Gene ID: 5596 Database links: Entrez Gene: 5596 Human Entrez Gene: 225724 Mouse Omim: 176949 Human SwissProt: P31152 Human SwissProt: Q6P5G0 Mouse Unigene: 433728 Human Unigene: 254517 Mouse Unigene: 146899 Rat MAP kinase 4（MAPK4）也是MAPK家族成員，具有絲/蘇氨酸激酶活性。參與細胞生長、增殖、分化、死亡及細胞間的功能同步等多種生理過程,能夠被諸如細胞因子、生長因子、神經遞質、激素、細胞應急以及細胞粘附等各種刺激因素激活為信號傳導途中重要的蛋白激酶，MAPK家族成員之間有很高的同源性。 |
| 產品圖片 |
Independently Validated Antibody, image provided by Science Direct, badge number 029727:Formalin-fixed and paraffin embedded human heart labeled with Rabbit Anti-ERK4 Polyclonal Antibody, Unconjugated (bs-1319R) at 1:250 overnight at 4C followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAPK4 Polyclonal Antibody, Unconjugated(bs-1319R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MAPK4) polyclonal Antibody, Unconjugated (bs-1319R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-MAPK4 antibody (bs-1319R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
