| 產品編號 | bs-0523R |
| 英文名稱 | Rabbit Anti-SOX2 antibody |
| 中文名稱 | 胚胎干細胞關鍵蛋白抗體 |
| 別 名 | transcriptional factor SOX2; ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; Sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; ysb; SOX2_HUMAN; Transcription factor SOX-2; SOX2_HUMAN. |
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Specific References (14) | bs-0523R has been referenced in 14 publications.
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| 研究領域 | 腫瘤 細胞生物 神經生物學 干細胞 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SOX2: 227-317/317 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]. Function: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Subunit: Interacts with ZSCAN10. Interacts with SOX3 and FGFR1. Subcellular Location: Nucleus. Post-translational modifications: Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. DISEASE: Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. Similarity: Contains 1 HMG box DNA-binding domain. SWISS: P48432 Gene ID: 6657 Database links: Entrez Gene: 6657 Human Entrez Gene: 20674 Mouse Omim: 184429 Human SwissProt: P48431 Human SwissProt: P48432 Mouse Unigene: 518438 Human Unigene: 65396 Mouse Embryonic Stem Cell Marker (胚胎干細胞標志物) 轉錄因子:胚胎干細胞相關蛋白Sox2是sox基因家族的一個成員,Sox2與Oct4、Nanog一樣是胚胎干細胞重要的轉錄因子，是維持干細胞特性中起到重要的作用因子；由于它在早期胚胎發生、神經分化和晶狀體發育等多種重要的發育事件中都起著關鍵的作用,從而引起了越來越廣泛的關注。 |
| 產品圖片 |
Sample:
Eye (Mouse) Lysate at 40 ug
Primary: Anti-SOX2 (bs-0523R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SOX2 (bs-0523R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Sample:
Eye(Rat) Lysate at 40 ug
Primary: Anti-SOX2 (bs-0523R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX2) Polyclonal Antibody, Unconjugated (bs-0523R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell: NCCIT
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-0523R) on NCCIT(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-SOX2 antibody (bs-0523R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: U251.
Primary Antibody (green line): Rabbit Anti-SOX2 antibody (bs-0523R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
