| 產品編號 | bs-0300R |
| 英文名稱 | Rabbit Anti-Mesothelin antibody |
| 中文名稱 | 間皮素抗體 |
| 別 名 | MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN. |
| 研究領域 | 腫瘤 免疫學 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64kDa |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mesothelin: 101-200/630 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor. Function: Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Subunit: Interacts with MUC16. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Megakaryocyte-potentiating factor: Secreted. Isoform 3: Secreted. Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). Post-translational modifications: Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. Similarity: Belongs to the mesothelin family. SWISS: Q13421 Gene ID: 10232 Database links: Entrez Gene: 10232 Human Omim: 601051 Human SwissProt: Q13421 Human Unigene: 408488 Human 間皮素（mesothelin, MSLN）是一種相對分子量：64kDa的細胞表面糖蛋白, 在間皮瘤、 卵巢癌、 胰腺癌等惡性腫瘤中有較高的表達。主要用于惡性腫瘤的研究。 |
| 產品圖片 |
Sample: Mcf-7 Cell Lysate at 40 ug
Primary: Anti- Mesothelin (bs-0300R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 62 kD
Blank control: Hela(blue). Primary Antibody: Rabbit Anti-Mesothelin/AF647 Conjugated antibody (bs-0300R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-0300R-AF647, 0.2μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
