| 產品編號 | bs-1082R |
| 英文名稱 | Rabbit Anti-GRK1 antibody |
| 中文名稱 | G蛋白偶合受體激酶1抗體 |
| 別 名 | G-protein coupled receptor kinase 1; GRK1; GPRK1; RK; Grk1; Rhok; RHODOPSIN KINASE; RK_HUMAN. |
| 研究領域 | 細胞生物 免疫學 神經生物學 信號轉導 激酶和磷酸酶 細胞膜受體 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Cow,Chicken) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRK1: 201-300/563 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq] Function: Phosphorylates rhodopsin thereby initiating its deactivation. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. Subcellular Location: Membrane. Tissue Specificity: Retina and pineal gland. Post-translational modifications: Autophosphorylated. Farnesylation is required for full activity. DISEASE: Defects in GRK1 are a cause of congenital stationary night blindness Oguchi type 2 (CSNBO2) [MIM:613411]. It is non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain. SWISS: Q15835 Gene ID: 6011 Database links: Entrez Gene: 6011 Human Entrez Gene: 24013 Mouse Omim: 180381 Human SwissProt: Q15835 Human SwissProt: Q9WVL4 Mouse Unigene: 103501 Human Unigene: 721727 Human Unigene: 257501 Mouse G蛋白偶聯受體激酶1(GRK1)是催化激動劑誘導的GPCR磷酸化以及啟動GPCR脫敏的關鍵激酶,主要用于GRK細胞膜轉位和對GRK功能的影響. |
| 產品圖片 |
Sample:
Lane 1: Human HUVEC cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human Jurkat cell lysates
Lane 4: Human Ramos cell lysates
Primary: Anti-GRK1 (bs-1082R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated(bs-1082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated(bs-1082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
