| 產品編號 | bs-0701R |
| 英文名稱 | Rabbit Anti-Ceramide glucosyltransferase antibody |
| 中文名稱 | 谷氨酸半胱氨酸γ合成酶抗體 |
| 別 名 | CEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Glucosylceramide synthase; UDP glucose ceramide glucosyltransferase; UDP glucose N acylsphingosine D glucosyltransferase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; Ugcg. |
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Specific References (3) | bs-0701R has been referenced in 3 publications.
[IF=4.936] Wang et al. TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity. (2017) Oxid.Med.Cell.Longev. 2017:4310475 WB ; Rat.
[IF=1.785] Jiannan Liu. et al. Prognostic value of using glucosylceramide synthase and cytochrome P450 family 1 subfamily A1 expression levels for patients with triple?negative breast cancer following neoadjuvant chemotherapy. Exp Ther Med. 2021 Mar;21(3):1-1 IHC ; Human.
[IF=1.49] Chen, Baoan, et al. "Effect of D, L?\threo?\1?\phenyl?\2?\decanoylamino?\3?\morpholino?\1?\propanol and tetrandrine on the reversion of multidrug resistance in K562/A02 cells."gy 16.1 (2011): 24-30..: 1064-1
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UGCG: 21-120/394 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GCS (glucosylceramide synthase ) may serve as a 'flippase' as well as a glucosyltransferase that transfers glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP-glucose. [Catalytic activity] UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Lipid metabolism; sphingolipid metabolism. [Subcellular location] Endoplasmic reticulum membrane; Multi-pass membrane protein. Belongs to the glycosyltransferase 2 family. Function: Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Found in all tissues examined. Similarity: Belongs to the glycosyltransferase 2 family. SWISS: Q16739 Gene ID: 7357 Database links: Entrez Gene: 7357 Human Entrez Gene: 22234 Mouse Omim: 602874 Human SwissProt: Q16739 Human SwissProt: O88693 Mouse Unigene: 304249 Human 谷氨酸半胱氨酸γ合成酶又稱葡萄糖神經酰胺合成酶(glucosylceramide synthase, GCS)是調控神經酰胺代謝的關鍵酶之一, 其活性水平與腫瘤細胞多藥耐藥有密切關系. 有研究報道GCS在人卵巢癌細胞、口腔表皮癌細胞 、黑色素瘤細胞、乳腺癌細胞及膽囊癌、胃癌中均有表達. |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GCS/glucosylceramide synthase Polyclonal Antibody, Unconjugated(bs-0701R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
